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fixable viability dye efluor 780  (Thermo Fisher)


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    Thermo Fisher fixable viability dye efluor 780
    Fixable Viability Dye Efluor 780, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fixable+viability+dye+efluor+780/PBS/bio_rxiv__2025__08__31__672068-227-13-18
    Average 99 stars, based on 1 article reviews
    fixable viability dye efluor 780 - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Recombinase Polymerase Amplification:

    Article Title: A Singular Base Editing Platform for Polyfunctional Multiplex Engineering of Immune Cells
    Article Snippet: .. The following antibodies, proteins, and dyes were used: PE- or BV421-conjugated anti-CD33 (clone WM53; BioLegend, San Diego, CA), PE-conjugated anti-mesothelin (clone REA1057; Miltenyi Biotec, Germany), PerCP-conjugated anti-CD19 (clone HIB19; BioLegend), PE- or FitC-conjugated anti-CD3 (clone SK7; BD Biosciences, Franklin Lakes, NJ), BV605-conjugated anti-CD4 (clone RPA-T4; BioLegend), PE-conjugate anti-CD34 to detect RQR8 (clone QBEND/10; Invitrogen, Carlsbad, CA), BV421-conjugated anti-PD-1 (clone EH12.2H7; BioLegend), PE-Cy7-conjugated anti-B2M (clone 2M2, BioLegend) BV650-conjugated anti-CD8 (clone RPA-T8; BioLegend), PE-Cy7-conjugated anti-IL-2 (clone MQ1-17H12; BioLegend), BV421-conjugated anti-IFNγ (clone 4S.B3; BioLegend), APC-conjugated anti-TNFɑ (clone Mab11; BioLegend), BV510-conjugated anti-CD107a (clone H4A3; BD Biosciences), Fixable viability dye eFluor 780 (eBioscience, Carlsbad, CA), Atto 647-conjugated recombinant human CD19 (BioTechne, Minneapolis, MN), Recombinant Human Siglec-3/CD33 Fc Chimera protein (R&D Systems), APC-conjugated anti-human Fc (clone QA10A42; BioLegend), anti-CISH mAb (clone D4D9, Cell Signaling Technologies, Boston, MA), and anti-actin (clone 8H10D10; Cell Signaling Technologies). .. Raji, HL-60, MOLM-13, OVCAR8, and K562 cells were acquired from ATCC.

    Recombinant:

    Article Title: A Singular Base Editing Platform for Polyfunctional Multiplex Engineering of Immune Cells
    Article Snippet: .. The following antibodies, proteins, and dyes were used: PE- or BV421-conjugated anti-CD33 (clone WM53; BioLegend, San Diego, CA), PE-conjugated anti-mesothelin (clone REA1057; Miltenyi Biotec, Germany), PerCP-conjugated anti-CD19 (clone HIB19; BioLegend), PE- or FitC-conjugated anti-CD3 (clone SK7; BD Biosciences, Franklin Lakes, NJ), BV605-conjugated anti-CD4 (clone RPA-T4; BioLegend), PE-conjugate anti-CD34 to detect RQR8 (clone QBEND/10; Invitrogen, Carlsbad, CA), BV421-conjugated anti-PD-1 (clone EH12.2H7; BioLegend), PE-Cy7-conjugated anti-B2M (clone 2M2, BioLegend) BV650-conjugated anti-CD8 (clone RPA-T8; BioLegend), PE-Cy7-conjugated anti-IL-2 (clone MQ1-17H12; BioLegend), BV421-conjugated anti-IFNγ (clone 4S.B3; BioLegend), APC-conjugated anti-TNFɑ (clone Mab11; BioLegend), BV510-conjugated anti-CD107a (clone H4A3; BD Biosciences), Fixable viability dye eFluor 780 (eBioscience, Carlsbad, CA), Atto 647-conjugated recombinant human CD19 (BioTechne, Minneapolis, MN), Recombinant Human Siglec-3/CD33 Fc Chimera protein (R&D Systems), APC-conjugated anti-human Fc (clone QA10A42; BioLegend), anti-CISH mAb (clone D4D9, Cell Signaling Technologies, Boston, MA), and anti-actin (clone 8H10D10; Cell Signaling Technologies). .. Raji, HL-60, MOLM-13, OVCAR8, and K562 cells were acquired from ATCC.

    other:

    Article Title: Simulating CD8 T cell exhaustion: A comprehensive approach
    Article Snippet: Viability was determined using a compatible dye (eBioscience Fixable Viability dye eFluor 780, eFluor 506, eFluor 455UV or DAPI).

    Staining:

    Article Title: Simulating CD8 T cell exhaustion: A comprehensive approach
    Article Snippet: Cells were seeded in a 96 well plate with CM media containing the protein transport inhibitor monensin (BD GolgiStop, 1 in 1000) and CD107a antibody (0.75 μg/mL), with or without OVA (2 μg/mL) for four hours at 37°C in 5% CO 2 . .. Cells were stained with eBioscience Fixable Viability dye eFluor 780 and fixed with Cytofix/Cytoperm solution for 20 min at 4°C. .. After washing with perm/wash solution (BD, USA) cells were stained for 30 min at 4°C.

    Article Title: Unknown Immunoregulatory Effects of FcRn Inhibition by Efgartigimod in Myasthenia Gravis
    Article Snippet: .. After extraction from fresh blood, PBMCs (1.5 × 106 cells) were stained with 1 μL/mL of Fixable Viability Dye eFluor 780 (Thermo Fisher Scientific) diluted in staining buffer, containing 1% phosphate-buffered saline, 2% bovine serum albumin (BSA), and 5mMEDTA, for 30minutes on ice in the dark. .. After centrifugation at 1,400 rpm for 7 minutes at 4°C and supernatant elimination, PBMCs were incubated on ice for 30 minutes with combinations of the following primary Abs (dilution 1:20): (1) mouse anti-CD19-Super Bright 702 (Invitrogen); (2) mouse anti-CD138/syndecan-1-PE (Invitrogen); (3) mouse anti-CD27-APC (Miltenyi); (4) mouse anti-CD45-Alexa Fluor 700 (Invitrogen); (5) mouse antiCD3-FITC (BD Biosciences); (6) mouse anti-CD4-APC- eFluor 780 (Invitrogen), and (7) mouse anti-CD8a-PerCPeFluor 710 (Invitrogen).

    Article Title: Glucose Metabolism Controls Oxidative Burst and Lipid Mediator Production in Neutrophils upon Microbial Challenge
    Article Snippet: .. In brief, cells were stained with fluorophore-conjugated antibodies ( Table S3 ) and Fixable Viability Dye eFluor 780 (eBioscience) for 15 min at RT in PBS. .. After washing with PBS containing 0.5% BSA, cells were fixed using the IC Fixation buffer (eBioscience) for 30 min. For intracellular staining, cells were washed with Permeabilization Buffer (eBioscience) according to manufacturer instructions and subsequently stained o/n with unconjugated antibodies at 4°C in permeabilization buffer (eBioscience).

    Extraction:

    Article Title: Unknown Immunoregulatory Effects of FcRn Inhibition by Efgartigimod in Myasthenia Gravis
    Article Snippet: .. After extraction from fresh blood, PBMCs (1.5 × 106 cells) were stained with 1 μL/mL of Fixable Viability Dye eFluor 780 (Thermo Fisher Scientific) diluted in staining buffer, containing 1% phosphate-buffered saline, 2% bovine serum albumin (BSA), and 5mMEDTA, for 30minutes on ice in the dark. .. After centrifugation at 1,400 rpm for 7 minutes at 4°C and supernatant elimination, PBMCs were incubated on ice for 30 minutes with combinations of the following primary Abs (dilution 1:20): (1) mouse anti-CD19-Super Bright 702 (Invitrogen); (2) mouse anti-CD138/syndecan-1-PE (Invitrogen); (3) mouse anti-CD27-APC (Miltenyi); (4) mouse anti-CD45-Alexa Fluor 700 (Invitrogen); (5) mouse antiCD3-FITC (BD Biosciences); (6) mouse anti-CD4-APC- eFluor 780 (Invitrogen), and (7) mouse anti-CD8a-PerCPeFluor 710 (Invitrogen).

    Saline:

    Article Title: Unknown Immunoregulatory Effects of FcRn Inhibition by Efgartigimod in Myasthenia Gravis
    Article Snippet: .. After extraction from fresh blood, PBMCs (1.5 × 106 cells) were stained with 1 μL/mL of Fixable Viability Dye eFluor 780 (Thermo Fisher Scientific) diluted in staining buffer, containing 1% phosphate-buffered saline, 2% bovine serum albumin (BSA), and 5mMEDTA, for 30minutes on ice in the dark. .. After centrifugation at 1,400 rpm for 7 minutes at 4°C and supernatant elimination, PBMCs were incubated on ice for 30 minutes with combinations of the following primary Abs (dilution 1:20): (1) mouse anti-CD19-Super Bright 702 (Invitrogen); (2) mouse anti-CD138/syndecan-1-PE (Invitrogen); (3) mouse anti-CD27-APC (Miltenyi); (4) mouse anti-CD45-Alexa Fluor 700 (Invitrogen); (5) mouse antiCD3-FITC (BD Biosciences); (6) mouse anti-CD4-APC- eFluor 780 (Invitrogen), and (7) mouse anti-CD8a-PerCPeFluor 710 (Invitrogen).

    Selection:

    Article Title: Purine nucleobases enhance CD8 + T cell effector function
    Article Snippet: Subsequently, 25 μL of PBS mixed with anti-CD16/32 (Biolegend; dilution 1:200) was added, and the mixture was incubated at room temperature for 10 minutes. .. Then, a 25 μL master mix containing a selection of surface marker antibodies (including anti-CD8.2b, anti-CD3 and others, all from Biolegend at a 1:200 dilution in PBS) along with Fixable Viability Dye eFluor 780 (eBioscience; 1:2000 in PBS) was added for a 20-minute incubation at 4°C. .. After washing with FACS buffer (PBS with 2% FCS), cells were fixed in 4% Paraformaldehyde (Sigma) in PBS for 10 minutes, washed twice more with FACS buffer, resuspended in 100 μL, and analyzed via flow cytometry.

    Marker:

    Article Title: Purine nucleobases enhance CD8 + T cell effector function
    Article Snippet: Subsequently, 25 μL of PBS mixed with anti-CD16/32 (Biolegend; dilution 1:200) was added, and the mixture was incubated at room temperature for 10 minutes. .. Then, a 25 μL master mix containing a selection of surface marker antibodies (including anti-CD8.2b, anti-CD3 and others, all from Biolegend at a 1:200 dilution in PBS) along with Fixable Viability Dye eFluor 780 (eBioscience; 1:2000 in PBS) was added for a 20-minute incubation at 4°C. .. After washing with FACS buffer (PBS with 2% FCS), cells were fixed in 4% Paraformaldehyde (Sigma) in PBS for 10 minutes, washed twice more with FACS buffer, resuspended in 100 μL, and analyzed via flow cytometry.

    Incubation:

    Article Title: Purine nucleobases enhance CD8 + T cell effector function
    Article Snippet: Subsequently, 25 μL of PBS mixed with anti-CD16/32 (Biolegend; dilution 1:200) was added, and the mixture was incubated at room temperature for 10 minutes. .. Then, a 25 μL master mix containing a selection of surface marker antibodies (including anti-CD8.2b, anti-CD3 and others, all from Biolegend at a 1:200 dilution in PBS) along with Fixable Viability Dye eFluor 780 (eBioscience; 1:2000 in PBS) was added for a 20-minute incubation at 4°C. .. After washing with FACS buffer (PBS with 2% FCS), cells were fixed in 4% Paraformaldehyde (Sigma) in PBS for 10 minutes, washed twice more with FACS buffer, resuspended in 100 μL, and analyzed via flow cytometry.



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